LOCUS       sequence-13060-e        3327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1166-HQ858710
FEATURES             Location/Qualifiers
     source          1..3327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(884..983)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1000..1019)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1001..1019)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1024..1040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1024..1040)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1142..1161)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1153..1962
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1220..1239)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2109..2697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2598..2617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2851..2868
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3027..3054)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3146..3232)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3281..3303
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3295..3312
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3296..3312
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggggttcc tcgaggactt ccaagccagt gttgaagcat tgccatctat gctgcatcgg
      181 aactattcat taatgcgaga gttggataaa agtttgcatg gtgtgcaact agaaaatgag
      241 cagcgctgtc aacaagaaat agaggacatt aagcatggac ttgaatctgg aagtattaca
      301 tatgacccag caaaattgaa attttccgaa gaagcaatgg aggagcagaa acattgtgtt
      361 agaattgctg acgagaaggt ggctctagcc actcagactt atgacttagt tgatgctcac
      421 atccaacagc tagatcagtt tatgaggaag cttgaagaaa tccggcaagg aaaagaggca
      481 gctgctgctg ttactgcagg gactgctgtt gctactactg ccactccaac cgtgaatgct
      541 ggagcgacaa ctgctgatgc taatccgaag agtggacgat ctggcgaaag aggcagaggg
      601 ggtcggaaga aggccaaggt acccacggag cagtcagtgc cagtgccagt gccactgcca
      661 cctattgact tagagttgcc tgtggatcct aacgaaccga catactgcct ctgcaaccaa
      721 gtcagctatg gtgagatggt ggcatgcgac aatcctaatt gcaaaatcga gtggtatcac
      781 tatggctgtg tgggcgtcaa ggagcaaccc aagggaaagt ggtattgccc aaactgcatc
      841 ggattccaga agaagcgaaa aggcaagaag ggtgggcgcg ccgacccagc tttcttgtac
      901 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
      961 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     1021 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     1081 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     1141 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     1201 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1261 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1321 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     1381 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1441 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1501 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1561 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1621 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     1681 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     1741 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     1801 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     1861 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     1921 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     1981 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     2041 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2101 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2161 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2221 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     2281 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2341 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2401 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2461 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     2521 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2581 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2641 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2701 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2761 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2821 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2881 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2941 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     3001 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     3061 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     3121 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     3181 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     3241 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     3301 acgacggcca gtcttaagct cgggccc
//
