LOCUS       sequence-130605-        9896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MosSCI-QUAS::Delta-pes10::GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Wei X, Potter CJ, Luo L, Shen K
  TITLE     Controlling gene expression with the Q repressible binary expression
            system in Caenorhabditis elegans.
  JOURNAL   Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
  PUBMED    22406855
REFERENCE   2  (bases 1 to 9896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "4"; pages: "391-5"
COMMENT     Sequence Label: XW82
FEATURES             Location/Qualifiers
     source          1..9896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1491..1502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4107..4135)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4883..4903
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(7941..7959)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8027..8131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8132..8200,8201..8992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8350..8369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9163..9751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9652..9671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa
       61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg
      121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt
      181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta
      241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt
      301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta
      361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa
      421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc
      481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa
      541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg
      601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc
      661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat
      721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca
      781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca
      841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga
      901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata
      961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga
     1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat
     1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca
     1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac
     1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc
     1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat
     1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt
     1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca acaggtttct
     2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3361 cgctaaaaac attaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac
     3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggtatctaga
     3541 accggtgacg tcaccatggg aagcttcgtg gatccagata tcctgcagga attcctcgag
     3601 acgtacggtg cgcgcgatgc attcgaagat ctgcccacta gggcatgcgg atccgggtaa
     3661 tcgcttatcc tcggataaac aattatcctc acgggtaatc gcttatccgc tcgggtaatc
     3721 gcttatcctc gggtaatcgc ttatccttaa gcttgatatc cctgcaggat cgattttttg
     3781 caaattacga gcgttgtagg gggcggagcg ataggtccta taggttttgg tatatcatca
     3841 ttcattcatt cattggtaca ttcatttacc caccttcctc tttctgagct tctctggagt
     3901 tctgtgcttc ctttttccct tatctttata ctgtaatttt taactttcag ggcgcgcctc
     3961 tagaggatcc ccgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata
     4021 acatagaaca ttttcaggag gacccttggc tagcgtcgac ggtaccggta gaaaaaatga
     4081 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
     4141 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
     4201 ttacccttaa atttatttgc actactggaa aactacctgt tccatgggta agtttaaaca
     4261 tatatatact aactaaccct gattatttaa attttcagcc aacacttgtc actactttct
     4321 gttatggtgt tcaatgcttc tcgagatacc cagatcatat gaaacggcat gactttttca
     4381 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     4441 actacaagac acgtaagttt aaacagttcg gtactaacta accatacata tttaaatttt
     4501 caggtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
     4561 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
     4621 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttgta agtttaaaca
     4681 tgattttact aactaactaa tctgatttaa attttcagaa cttcaaaatt agacacaaca
     4741 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     4801 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     4861 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     4921 atggcatgga tgaactatac aaatagcatt cgtagaattc caactgagcg ccggtcgcta
     4981 ccattaccaa cttgtctggt gtcaaaaata ataggggccg ctgtcatcag agtaagttta
     5041 aactgagttc tactaactaa cgagtaatat ttaaattttc agcatctcgc gcccgtgcct
     5101 ctgacttcta agtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc
     5161 accccctatt tttgttatta tcaaaaaaac ttcttcttaa tttctttgtt ttttagcttc
     5221 ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
     5281 aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
     5341 cacaatgttc tgtgtacact tcttatgttt tttttacttc tgataaattt tttttgaaac
     5401 atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg
     5461 caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa
     5521 aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc
     5581 ctgcttttgc tttttggggg tttcccctat tgtttgtcaa gagtttcgag gacggcgttt
     5641 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     5701 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     5761 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     5821 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     5881 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     5941 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     6001 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     6061 gtaaggagaa aataccgcat caggcggcct taattaatct agtgagtcgt attataagtg
     6121 caagtaagat cagtgtttgt ttcgatatca gtctgtttcg taacggtctt ctgtataact
     6181 acaaaaaaga ataaaaaacc gtatcacaac tgagaaaaaa gacaaaactt attgaacagt
     6241 gttcctttca aaaaattatt ttgtaaaaaa tcgagttttt ctccaaaata acgcacgcaa
     6301 tcaaaaacaa gaaaactaac gtgttctcct gggcgtggaa ctccttatca agattaccat
     6361 tattgtctgt cagaattgat atttcaatta tttcatatct agacaaaccc gggtgtcttg
     6421 catttattag tactgtgagt ttacatacaa acgaggctta ctcagataac atagagtcac
     6481 ctctaattag caaaaaccca tttctaatta gctatttcga ccagttctaa agtcaccgta
     6541 attagaaatg gtttttgtag cttttctaat tagaaatgac cccgagataa gtgaaatatg
     6601 gcattttctg ggttttctag taagaaatga tcccgcacgt agtgattccg cgttacaagc
     6661 caaattcaaa ttaaaaaagt atattacaca attcaagtta cattattttc aggaaacaat
     6721 ggcgaagagc aaagcagaga ttcaaaagga ggaaagagac aacagatatg aacgaagact
     6781 tcttgaaaag agaaagagac atgaggtgag taaccatgat aataaataat caagtaaggg
     6841 ctatagattt ataaattctg gagttaacag tttcgaaaaa aagtagtttt catatgtgcg
     6901 aaggaatatc aaaaataggg gaatagccta aacgagtgag aaaattaaat gtttgttatt
     6961 ttttcaaaaa tgtctgcagg gccgttatct tttcaaatgc actctatcaa acatagatca
     7021 tatccatcat atcttatcca gaatttatat catatccaag attgagagcg tttcaaggaa
     7081 aatctcaaac aaccatacaa ttttgtagat cgacaacaag gcctccactt cttcatcgat
     7141 ggcctctgca gcacctgcac caccaagttt gtatttttca tattaatata aagtgagcca
     7201 cgcatagtcg tttttaggat ttttgccaga tcgatgtcgc gcaaacagac tgtttttttt
     7261 tctttgaaag ccgtgcgtga catgggccac ggattgtaac gccgcgctgg atggataaaa
     7321 atttattcag ttttttagcc tttacaaaat atggttcgac tactttcttt atttttacat
     7381 ttttataggt gctcattgag ctgagatatt ttgggtataa tcggaaaatc gctgattttg
     7441 gtcatttttc tgcactaaag attacacaaa aagttcagta tttagattca ttgttgaaaa
     7501 ttggggatga tctacagata catatcgccg tgttcaaaaa cacacgtgct gtgacgtcgt
     7561 gactggggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     7621 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     7681 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     7741 ccgcatatgg tgcactctca gtgcaatctg ctctgatgcc gcatagttaa gccagccccg
     7801 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     7861 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     7921 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     7981 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8041 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8101 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8161 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8221 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8281 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8341 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8401 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8461 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8521 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8581 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8641 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8701 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8761 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8821 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8881 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8941 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9001 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9061 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9121 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9181 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9241 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9301 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9361 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9421 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9481 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9541 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9601 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9661 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9721 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9781 cctggtcttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9841 ggataaccgt attaccgcct ttgagtgagc tgataccagc ggataacaat ttcaca
//
