LOCUS       sequence-130607-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  redox-sensitive red fluorescent protein rxRFP1.4, endoplasmic 
            reticulum localized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Fan Y, Ai HW
  TITLE     Development of redox-sensitive red fluorescent proteins for imaging 
            redox dynamics in cellular compartments.
  JOURNAL   Anal Bioanal Chem. 2016 Jan 12.
  PUBMED    26758595
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Bioanal Chem. 2016 Jan 12."
COMMENT     Sequence Label: pER-rxRFP1.4
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..84
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..120
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             133..147
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     891..910
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aagcttatgc tgctatccgt gcccctgctg ctcggcctgc tgggcctggc cgccgccgac
       61 ggatcccatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
      121 cgggatccgt acgacgatga cgataaggat ccgagctcga gctctcctcg ttgcgctata
      181 ggtcggctga gctcacccgt ggtttccgag cggatgtacc ccgaggacgg cgccctgaag
      241 agcgagatca agaaggggct gaggctgaag gacggcggcc actacgccgc cgaggtcaag
      301 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt cgacatcaag
      361 ttggacatcg tgtcccacaa cgaggactac accatcgtgg aacagtgcga acgcgccgag
      421 ggccgccact ccaccggcgg catggacgag ctgtacaagg gaggtacagg cgggagtctg
      481 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
      541 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      601 ccctacgagg cctttcagac cgctaagctg aaggtgacca agggtggccc cctgcccttc
      661 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacat taagcaccca
      721 gccgacatcc ccgactactt caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg
      781 atgaacttcg aggacggcgg cattattcac gttaaccagg actcctccct gcaggacggc
      841 gtattcatct acaaggtgaa gctgcgcggc accaacttcc cccccgacgg ccccgtaatg
      901 cagaagaaga ccatgggctg ggaggctacg cgtgaccaaa ggtgcagtcg taagcttgga
      961 ggttctggag gaaaggacga gctgtaatct aga
//
