LOCUS       sequence-130612-         459 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Temperature sensitive plasmid for either lambda Red recombinase 
            genes or flippase recombinase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Jensen SI, Lennen RM, Herrgard MJ, Nielsen AT
  TITLE     Seven gene deletions in seven days: Fast generation of Escherichia 
            coli strains tolerant to acetate and osmotic stress.
  JOURNAL   Sci Rep. 2015 Dec 8;5:17874. doi: 10.1038/srep17874.
  PUBMED    26643270
REFERENCE   2  (bases 1 to 459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-12-8"; volume: "5:17874. doi"; pages: " 
            10.1038/srep17874"
COMMENT     Sequence Label: pSIJ8
FEATURES             Location/Qualifiers
     source          1..459
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgccacaat ttggtatatt atgtaaaaca ccacctaagg tgcttgttcg tcagtttgtg
       61 gaaaggtttg aaagaccttc aggtgagaaa atagcattat gtgctgctga actaacctat
      121 ttatgttgga tgattacaca taacggaaca gcaatcaaga gagccacatt catgagctat
      181 aatactatca taagcaattc gctgagtttc gatattgtca ataaatcact ccagtttaaa
      241 tacaagacgc aaaaagcaac aattctggaa gcctcattaa agaaattgat tcctgcttgg
      301 gaatttacaa ttattcctta ctatggacaa aaacatcaat ctgatatcac tgatattgta
      361 agtagtttgc aattacagtt cgaatcatcg gaagaagcag ataagggaaa tagccacagt
      421 aaaaaaatgc ttaaagcact tctaagtgag ggtgaaagc
//
