LOCUS       sequence-130613-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Temperature sensitive plasmid for either lambda Red recombinase 
            genes or flippase recombinase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Jensen SI, Lennen RM, Herrgard MJ, Nielsen AT
  TITLE     Seven gene deletions in seven days: Fast generation of Escherichia 
            coli strains tolerant to acetate and osmotic stress.
  JOURNAL   Sci Rep. 2015 Dec 8;5:17874. doi: 10.1038/srep17874.
  PUBMED    26643270
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-12-8"; volume: "5:17874. doi"; pages: " 
            10.1038/srep17874"
COMMENT     Sequence Label: pSIJ8
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagcccgaca tctgctacgc cacagcgagg ccagcgttac tgacatcgcc tatcgctgtg
       61 gattcagcga cagtaaccac ttttcgacgc tttttcgccg agagtttaac tggtcaccgc
      121 gtgatattcg ccagggacgg gatggctttc tgcaataacg cgaatcttct caacgtattt
      181 gtacgccata ttgcgaataa tcaacttcgt tctctggccg aggtagccac ggtggcgcat
      241 cagttaaaac ttctcaaaga tgattttttt gccagcgacc agcaggcagt cgctgtggct
      301 gaccgttatc cgcaagatgt ctttgctgaa catacacatg atttttgtga gctggtgatt
      361 gtctggcgcg gtaatggcct gcatgtactc aacgatcgcc cttatcgcat tacccgtggc
      421 gatctctttt acattcatgc tgacgataaa cactcctacg cttccgttaa cgatctggtt
      481 ttgcagaata ttatttattg cccggagcgt ctgaagctga atcttgactg gcagggggcg
      541 attccgggat ttaacgccag cgcagggcaa ccacactggc gcttaggtag catggggatg
      601 gcgcaggcgc ggcaggttat cggtcagctt gagtatgaaa gtagtcagca tgtgccgttt
      661 gctaacgaaa tggctgagtt gctgttcggg cagttggtga tgttgctgaa tcgccatcgt
      721 tacaccagtg attcgttgcc gccaacatcc agcgaaacgt tgctggat
//
