LOCUS       sequence-130620-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-catenin reporter - 3rd generation self-inactivating lentiviral 
            plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, 
            half-life of 2h)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Reya T, Duncan AW, Ailles L, Domen J, Scherer DC, Willert K, Hintz 
            L, Nusse R, Weissman IL
  TITLE     A role for Wnt signalling in self-renewal of haematopoietic stem 
            cells.
  JOURNAL   Nature. 2003 May 22. 423(6938):409-14.
  PUBMED    12717450
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 May 22. 423(6938):409-14."
COMMENT     Sequence Label: Lentiviral-TOP-dGFP-reporter
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(61..183,184..189,190..900,901..903,
                     904..906))
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(232..253)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     581..600
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     840..861
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      903..912
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      928..937
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt gtcgacctgc aggcggccgc
       61 ctacacattg atcctagcag aagcacaggc tgcagggtga cggtccatcc cgctctcctg
      121 ggcacaagac atgggcagcg tgccatcatc ctgctcctcc acctccggcg ggaagccatg
      181 gctaagcttc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      241 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
      301 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      361 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
      421 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
      481 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
      541 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
      601 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
      661 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
      721 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
      781 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
      841 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
      901 caccatggtg gcgaccggtg gatcatccca tggtggcttt accaacagta ccggaatgcc
      961 aagcttactt agatcgcaga tctcgagtac cggatccatt atataccctc tagacctttg
     1021 atcttacccc
//
