LOCUS       sequence-130627-        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP588-1
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..55
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             56..76
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             77..787
                     /codon_start=1
                     /product="monomeric green fluorescent protein, also known 
                     as mTagGFP"
                     /label=TagGFP2
                     /note="mammalian codon-optimized"
                     /translation="SGGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEIKF
                     ICTTGKLPVPWPTLVTTLCYGIQCFARYPEHMKMNDFFKSAMPEGYIQERTIQFQDDGK
                     YKTRGEVKFEGDTLVNRIELKGKDFKEDGNILGHKLEYSFNSHNVYIRPDKANNGLEAN
                     FKTRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSTQTKISKDRNEARDHMVLLES
                     FSACCHTHGMDELYR"
     CDS             788..808
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             809..874
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(922..938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(922..938)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(935..957)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    946..962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(970..976,977..994,995..1000))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1015..1036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aattcgagct cggtacccgg ggatcgaaca aaaactgata tcagaagagg atctggaaaa
       61 tttatatttt cagggtagcg ggggcgagga gctgttcgcc ggcatcgtgc ccgtgctgat
      121 cgagctggac ggcgacgtcc acggacacaa gttctccgtc cgcggagagg gagagggaga
      181 cgccgactac ggaaagctgg agatcaagtt catctgcacc accggcaagc tgcccgtgcc
      241 ctggcccacc ctagtgacca ccctctgcta cggcatccag tgcttcgccc gctaccccga
      301 gcacatgaag atgaacgact tcttcaagag cgccatgccc gagggctaca tacaggagcg
      361 caccatccag ttccaggacg acggcaagta caagacccgc ggcgaggtga agttcgaggg
      421 cgacaccctg gtgaaccgca tagagctgaa gggcaaggac ttcaaggagg acggcaacat
      481 cctgggccac aagctggagt acagcttcaa cagccacaat gtgtacatcc gccccgacaa
      541 ggccaacaac ggcctggagg ctaacttcaa gacccgccac aacatcgagg gcggcggcgt
      601 gcagctagcc gaccactacc agaccaacgt gcccctgggc gacggccccg tgctaatccc
      661 catcaaccac tacctgagca ctcagaccaa gatcagcaag gatcgcaacg aggcccgcga
      721 ccacatggtg ctcctggagt ccttcagcgc ctgctgccac acccacggca tggacgagct
      781 gtacagggag aacctctact tccaaggaga ctacaaagac catgacggtg attataaaga
      841 tcatgatatc gattacaagg atgacgatga caaggatcct ctagagtcga cctgcaggca
      901 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      961 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1021 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1081 gtgccagctg cattaatgaa tcggccaacg cgcggg
//
