LOCUS       sequence-130628-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP588-1
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..115)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(116..136)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(137..847)
                     /codon_start=1
                     /product="monomeric green fluorescent protein, also known 
                     as mTagGFP"
                     /label=TagGFP2
                     /note="mammalian codon-optimized"
                     /translation="SGGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEIKF
                     ICTTGKLPVPWPTLVTTLCYGIQCFARYPEHMKMNDFFKSAMPEGYIQERTIQFQDDGK
                     YKTRGEVKFEGDTLVNRIELKGKDFKEDGNILGHKLEYSFNSHNVYIRPDKANNGLEAN
                     FKTRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSTQTKISKDRNEARDHMVLLES
                     FSACCHTHGMDELYR"
     CDS             complement(848..868)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(869..898)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(925..942)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(925..941)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(934..956)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaggatcc ttgtcatcgt
       61 catccttgta atcgatatca tgatctttat aatcaccgtc atggtctttg tagtctcctt
      121 ggaagtagag gttctccctg tacagctcgt ccatgccgtg ggtgtggcag caggcgctga
      181 aggactccag gagcaccatg tggtcgcggg cctcgttgcg atccttgctg atcttggtct
      241 gagtgctcag gtagtggttg atggggatta gcacggggcc gtcgcccagg ggcacgttgg
      301 tctggtagtg gtcggctagc tgcacgccgc cgccctcgat gttgtggcgg gtcttgaagt
      361 tagcctccag gccgttgttg gccttgtcgg ggcggatgta cacattgtgg ctgttgaagc
      421 tgtactccag cttgtggccc aggatgttgc cgtcctcctt gaagtccttg cccttcagct
      481 ctatgcggtt caccagggtg tcgccctcga acttcacctc gccgcgggtc ttgtacttgc
      541 cgtcgtcctg gaactggatg gtgcgctcct gtatgtagcc ctcgggcatg gcgctcttga
      601 agaagtcgtt catcttcatg tgctcggggt agcgggcgaa gcactggatg ccgtagcaga
      661 gggtggtcac tagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttga
      721 tctccagctt tccgtagtcg gcgtctccct ctccctctcc gcggacggag aacttgtgtc
      781 cgtggacgtc gccgtccagc tcgatcagca cgggcacgat gccggcgaac agctcctcgc
      841 ccccgctacc ctgaaaatat aaattttcca gatcctcttc tgatatcagt ttttgttcga
      901 tccccgggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
      961 ccctggcgtt acccaactta atcgccttgc a
//
