LOCUS       sequence-13063-e        3465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1169-HQ858713
FEATURES             Location/Qualifiers
     source          1..3465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1022..1121)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1138..1157)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1139..1157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1162..1178)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1162..1178)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1280..1299)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1291..2100
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1358..1377)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2247..2835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2736..2755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2989..3006
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3165..3192)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3284..3370)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3419..3441
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3433..3450
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3434..3450
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgagcggcg ccggtccgga tctgcagctg ccaccggggt tccggttcca cccgacggac
      181 gaggagctgg tgatgcacta cctctgccgc cgctgcgccg gcctgcccat cgccgtcccc
      241 atcatcgccg agatcgacct ctacaagttc gacccatggc agctcccaag gatggcgctg
      301 tacggcgaga aggagtggta cttcttctcc ccgcgggacc gcaagtaccc gaacgggtcc
      361 aggcccaacc gcgccgccgg ggctgggtac tggaaggcca ccggcgctga caagcccgtg
      421 ggcacgccca agccgctggc catcaagaag gcgctcgtct tctacgccgg caaggcgccc
      481 aagggcgaga agaccaactg gatcatgcac gagtaccgcc tcgccgacgt cgaccgctcg
      541 gcgcgcaaga agaacagcct caggttggat gactgggtcc tgtgccgcat ctacaacaag
      601 aagggcggcg ggctggagaa ggcggcggcg ccggcggccg gcggcgacca caagcctgtg
      661 ttcgccacgg cggcggtgag ctccccgccg gagcagaagc cgttcgtggc ggcggcgggc
      721 gggctgcccc cggcgttccc ggagctggcg gcgtactacg accggccgtc ggactcgatg
      781 ccgcggctgc acgcggacta ctccagctgc tcggagcagg tgctgtcccc ggagcagctg
      841 gcgtgcgacc gggaggtgca gagccagccc aagatcagcg agtgggagcg gaccttcgcc
      901 tccgaccccg tgaaccccgc gggctccatg ctcgaccccg tcgtcggcca cgccggcggc
      961 gacccgctgc tgcaggacat cctcatgtac tggggcaagc cgttcaaggg tgggcgcgcc
     1021 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     1081 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     1141 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1201 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1261 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1321 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     1381 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     1441 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     1501 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     1561 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     1621 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     1681 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     1741 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     1801 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     1861 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     1921 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     1981 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2041 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2101 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2161 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     2401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     2641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2941 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3001 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3061 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3121 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3181 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3241 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3301 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3361 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3421 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
