LOCUS       sequence-13065-e        3585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1171-HQ858715
FEATURES             Location/Qualifiers
     source          1..3585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1142..1241)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1258..1277)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1259..1277)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1282..1298)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1282..1298)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1400..1419)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1411..2220
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1478..1497)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2367..2955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2856..2875
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3109..3126
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3285..3312)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3404..3490)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3539..3561
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3553..3570
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3554..3570
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggcagatg ctagttcgag gactgacacc tcgactgttg tagacatgga tgataagaat
      181 caaaggttag aaaacggaca gagtggagcc atggtcccgg cttctaattc atctgaccgg
      241 tctgacagat ctgacaaacc tatggaccag aaggttttac gacggcttgc ccaaaatcgt
      301 gaggcagcaa gaaaaagccg gctgaggaaa aaggcatatg tgcaacagct agagagcagt
      361 aagctaaaac ttgcaagcct ggagcaagaa ctccagaaag ctcgacagca gggaatcttc
      421 atttctagtt ctggagatca aactcatact atgagtggaa atggagctat gaccttcgat
      481 ttagaatatt ctcgatggca agaggaacaa aataagcaga taaatgagct gaggactgca
      541 gtaaatgctc atgcaagtga tagtgacctc cgtcttatag tcgatgggat aatggcacat
      601 tatgacgaga tattcaggct gaagggcatt gctgcaaagg ctgatgtgtt ccatatactt
      661 tcaggcatgt ggaaaacatc tgctgaaagg tgcttcttgt ggcttggggg tttccgttcg
      721 tctgagcttc taaagctcct tgtgaatcag cttgagcctc taactgagca gcaattgatg
      781 gggctgtcca atctgcagca atcttctcag caggctgagg atgcattgtc acaaggaatg
      841 gaagcattgc agcagtcctt ggcagaaacg ctggctgggt cccttggtcc atcaggatct
      901 tctggaaacg tggcaaacta tatgggtcaa atggctatgg ccatgggaaa acttggcaca
      961 ctcgagaatt tccttcgcca ggctgacaat ctacgacagc agaccttaca tcagatgcaa
     1021 cgtatcctga caatccgaca agctgctcgt gcgcttcttg caatccatga ctacttttca
     1081 cgtttgcgcg cactgagttc tctttggctt gctaggccac gagagaaggg tgggcgcgcc
     1141 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     1201 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     1261 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1321 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1381 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1441 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     1501 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     1561 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     1621 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     1681 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     1741 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     1801 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     1861 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     1921 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     1981 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2041 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2101 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2161 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2221 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2281 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2341 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2401 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2461 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     2521 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2581 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2641 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2701 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     2761 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2821 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2881 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2941 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3001 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3061 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3121 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3181 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3241 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3301 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3361 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3421 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3481 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3541 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
