LOCUS       sequence-130652-         567 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate AAV virus that will express tdTomato in the 
            presence of Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Oh SW, Harris JA, Ng L, Winslow B, Cain N, Mihalas S, Wang Q, Lau C,
            Kuan L, Henry AM, Mortrud MT, Ouellette B, Nguyen TN, Sorensen SA, 
            Slaughterbeck CR, Wakeman W, Li Y, Feng D, Ho A, Nicholas E, 
            Hirokawa KE, Bohn P, Joines KM, Peng H, Hawrylycz MJ, Phillips JW, 
            Hohmann JG, Wohnoutka P, Gerfen CR, Koch C, Bernard A, Dang C, Jones
            AR, Zeng H
  TITLE     A mesoscale connectome of the mouse brain.
  JOURNAL   Nature. 2014 Apr 10;508(7495):207-14. doi: 10.1038/nature13186. Epub
            2014 Apr 2.
  PUBMED    24695228
REFERENCE   2  (bases 1 to 567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13186"; journalName: "Nature"; date: "2014-04-10- 
            10"; volume: "508"; issue: "7495"; pages: "207-14"
COMMENT     Sequence Label: AAV pCAG-FLEX-tdTomato-WPRE
FEATURES             Location/Qualifiers
     source          1..567
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(38..71)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(82..115)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      146..155
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(285..303)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tccagaggtt gattatcgat aagcttgatg atcttcgata acttcgtata ggatacttta
       61 tacgaagtta ttgcgattcg gataacttcg tatagcatac attatacgaa gttatcaagg
      121 ccggccaggt tggcgcgccg ctagcgccac catggtgagc aagggcgagg aggtcatcaa
      181 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
      241 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      301 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
      361 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
      421 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
      481 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
      541 cccccccgac ggccccgtaa tgcagaa
//
