LOCUS       sequence-130660-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Optogenetic PI3K activation system; cytoplasmic component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Toettcher JE, Gong D, Lim WA, Weiner OD
  TITLE     Light-based feedback for controlling intracellular signaling 
            dynamics.
  JOURNAL   Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
  PUBMED    21909100
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-09-11- 11"; volume: "8"; 
            issue: "10"; pages: "837-9"
COMMENT     Sequence Label: pHR iSH-YFP-PIF
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat tctcacgcgt
       61 gccaccatgg catccaagta ccaacaagac caggtggtga aggaggacag cgtagaggct
      121 gtgggcgccc agctcaaggt ctaccaccag cagtaccagg acaagagccg cgaatatgac
      181 cagctgtatg aagaatacac acggacctcc caggagctgc agatgaagcg cacagccata
      241 gaggccttca acgagaccat caagatcttc gaagagcagg gccagacaca ggagaagtgc
      301 agcaaggagt atttggagcg cttccggcga gagggaaatg agaaggagat gcagaggatc
      361 ctgctgaact ccgagcgact caagtctcgc atcgcggaga tacacgaaag ccgcacgaag
      421 ttggagcagg atctgcgggc gcaggcctcc gacaaccgtg agatcgacaa gcgcatgaac
      481 agcctcaaac ctgacctcat gcagctgcgc aagatcaggg accagtacct cgtgtggctc
      541 acccagaaag gtgcccgaca gaggaagatc aacgaatggc tgggaatcaa gaacgagact
      601 gaggaccagt attcactgat ggaggatgag gacgccctcc cccaccacga ggagcgcacg
      661 gaattcagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct
      721 ggtggtgcta gcatggtgag caagggcgag gagctgttca ccgg
//
