LOCUS       sequence-130665-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Optogenetic Ras activation system; cytoplasmic component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Toettcher JE, Weiner OD, Lim WA
  TITLE     Using optogenetics to interrogate the dynamic control of signal 
            transmission by the ras/erk module.
  JOURNAL   Cell. 2013 Dec 5;155(6):1422-34. doi: 10.1016/j.cell.2013.11.004.
  PUBMED    24315106
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.11"; journalName: "Cell"; date: "2013-12-5- 5";
            volume: "155"; issue: "6"; pages: "1422-34"
COMMENT     Sequence Label: pHR SFFVp BFP-PIF-SOS2cat
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..800
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgtc
       61 aagtggagca aggcaggtgg acagtggatc cgcttggtac catgagcgag ctgattaagg
      121 agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca
      181 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
      241 agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca
      301 agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg
      361 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
      421 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca
      481 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc
      541 tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg
      601 ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc gctaagaacc
      661 tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca
      721 acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca
      781 aactggggca caagcttaat ggatccatga tgttcttacc aaccgattat tgttgcaggt
      841 taagcgatca agagtatatg gagcttgtgt
//
