LOCUS       sequence-130666-         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Zhu G, Wu CJ, Zhao Y, Ashwell JD
  TITLE     Optineurin negatively regulates TNFalpha- induced NF-kappaB 
            activation by competing with NEMO for ubiquitinated RIP.
  JOURNAL   Curr Biol. 2007 Aug 21. 17(16):1438-43.
  PUBMED    17702576
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2007 Aug 21. 17(16):1438-43."
COMMENT     Sequence Label: pDEST26-hOPTN
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             131..148
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    158..182
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             517..528
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccggactc tagcctaggc cgcggaccat
      121 ggcgtactac catcaccatc accatcactc tagatcaaca agtttgtaca aaaaagcagg
      181 ctccgcggcc gcccccttca cctcccatca acctctcagc tgcctcactg aaaaggagga
      241 cagccccagt gaaagcacag gaaatggacc cccccacctg gcccacccaa acctggacac
      301 gtttaccccg gaggagctgc tgcagcagat gaaagagctc ctgaccgaga accaccagct
      361 gaaagaagcc atgaagctaa ataatcaagc catgaaaggg agatttgagg agctttcggc
      421 ctggacagag aaacagaagg aagaacgcca gttttttgag atacagagca aagaagcaaa
      481 agagcgtcta atggccttga gtcatgagaa tgagaaattg aaggaagagc ttggaaaact
      541 aaaagggaaa tcagaaaggt catctgagga ccccactgat gactccaggc ttcccagggc
      601 cgaagcggag caggaaaagg accagctcag gacccaggtg gtgaggctac aagcagagaa
      661 ggcagacctg ttgg
//
