LOCUS       sequence-130667-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for membrane PIP3 levels.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Toettcher JE, Gong D, Lim WA, Weiner OD
  TITLE     Light-based feedback for controlling intracellular signaling 
            dynamics.
  JOURNAL   Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
  PUBMED    21909100
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-09-11- 11"; volume: "8"; 
            issue: "10"; pages: "837-9"
COMMENT     Sequence Label: pHR SFFVp PHAkt Cerulean
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(630..651)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagtcctccg acagactgag tcgcccgggg gggatctgga gctctcgaga attctcacgc
       61 gtgccaccat gagcgacgtg gctattgtga aggagggttg gctgcacaaa cgaggggagt
      121 acatcaagac ctggcggcca cgctacttcc tcctcaagaa tgatggcacc ttcattggct
      181 acaaggagcg gccgcaggat gtggaccaac gtgaggctcc cctcaacaac ttctctgtgg
      241 cgcagtgcca gctgatgaag acggagcggc cccggcccaa caccttcatc atccgctgcc
      301 tgcagtggac cactgtcatc gaacgcacct tccatgtgga gactcctgag gagcgggagg
      361 agtggacaac cgccatccag actgtggctg acggcctcaa gaagcaggag gaggaggaga
      421 tggacttccg gtcgggctca cccagtgaca actcaggggc tgaagagatg gaggtgtccc
      481 tggccaagcc caagcaccgc gtgaccatga acgagtttga gtacctgaag ctgctgggca
      541 agggcacttt cggcaaggta cctagcggcc atatggggcc aagaatggtg agcaagggcg
      601 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      661 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      721 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      781 cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
      841 agtccgccat gcc
//
