LOCUS       sequence-130672-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Winslow MM, Dayton TL, Verhaak RG, Kim-Kiselak C, Snyder EL, Feldser
            DM, Hubbard DD, DuPage MJ, Whittaker CA, Hoersch S, Yoon S, Crowley 
            D, Bronson RT, Chiang DY, Meyerson M, Jacks T
  TITLE     Suppression of lung adenocarcinoma progression by Nkx2-1.
  JOURNAL   Nature. 2011 May 5. 473(7345):101-4.
  PUBMED    21471965
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 May 5. 473(7345):101-4."
COMMENT     Sequence Label: pLKO.shHmga2
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    140..257
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        306..815
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     735..753
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 cgatttcttg gctttatata tcttgtggaa aggacgaggt accgggaaac ttatcaagac
       61 gattaactcg agttaatcgt cttgataagt ttctttttga attctagatc ttgagacaaa
      121 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
      181 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
      241 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
      301 tcgagggggc ccgggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
      361 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
      421 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
      481 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
      541 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
      601 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
      661 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
      721 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
      781 tccctcgttg accgaatcac cgacctctct cccca
//
