LOCUS       sequence-130673-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used as a template for in vitro transcription of Cas9 nickase mRNA 
            after linearizing with BbsI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Karl Degenhardt
  TITLE     Degenhardt lab Cas9 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TC9nickpAB
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtcggtatcc acggagtccc agcagccgac aagaagtaca gcatcggcct ggccatcggc
       61 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
      121 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
      181 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
      241 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
      301 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
      361 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
      421 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg
      481 ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc
      541 gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac
      601 aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg
      661 tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag
      721 aagaagaatg gcctgttcgg caacctgatt gccctgagcc tgg
//
