LOCUS       sequence-130675-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP
            and shRNA to be recombined out of the construct, turning OFF shRNA 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L,
            Jaenisch R, Jacks T
  TITLE     Cre-lox-regulated conditional RNA interference from transgenes.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.
  PUBMED    15240889
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jul 13. 101(28):10380-5."
COMMENT     Sequence Label: pSicoR
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(67..270)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(96..116)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        271..574
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(767..787)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtagcgc tagcggatct gacggttcac
       61 taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      121 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      181 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      241 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      301 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      361 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      421 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      481 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
      541 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
      601 taatgacccc gtaattgatt actattaata actaatgcat ggcggtaata cggttatcca
      661 cgcggccgcc taatggatcc tagtactcga gggtcaccgc gcgttaacaa ggcttttctc
      721 caagggatat ttatagtctc aaaacacaca attactttac agttagggtg agtttccttt
      781 tgtgctgttt ataacttcg
//
