LOCUS       sequence-130676-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Prickett TD, Wei X, Cardenas-Navia I, Teer JK, Lin JC, Walia V, 
            Gartner J, Jiang J, Cherukuri PF, Molinolo A, Davies MA, Gershenwald
            JE, Stemke-Hale K, Rosenberg SA, Margulies EH, Samuels Y
  TITLE     Exon capture analysis of G protein-coupled receptors identifies 
            activating mutations in GRM3 in melanoma.
  JOURNAL   Nat Genet. 2011 Sep 25;43(11):1119-26. doi: 10.1038/ng.950.
  PUBMED    21946352
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng"; 
            journalName: "Nat Genet"; date: "2011-09-25- 25"; volume: "43"; 
            issue: "11"; pages: "1119-26"
COMMENT     Sequence Label: pCDF1-GRM3 (G561E)
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             438..461
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        506..717
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             730..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
ORIGIN
        1 gattcagcat gttgatctct cttacctacg atgtgatcct ggtgatctta tgcactgtgt
       61 acgccttcaa aacgcggaag tgcccagaaa atttcaacga agctaagttc ataggtttta
      121 ccatgtacac cacgtgcatc atctggttgg ccttcctccc tatattttat gtgacatcaa
      181 gtgactacag agtgcagacg acaaccatgt gcatctctgt cagcctgagt ggctttgtgg
      241 tcttgggctg tttgtttgca cccaaggttc acatcatcct gtttcaaccc cagaagaatg
      301 ttgtcacaca cagactgcac ctcaacaggt tcagtgtcag tggaactggg accacatact
      361 ctcagtcctc tgcaagcacg tatgtgccaa cggtgtgcaa tgggcgggaa gtcctcgact
      421 ccaccacctc atctctggat tacaaggatg acgacgataa gtaggcggcc gcgaaggatc
      481 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
      541 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
      601 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
      661 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
      721 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
      781 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
      841 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
      901 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
      961 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc gg
//
