LOCUS       sequence-13068-e        3534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1175-HQ858716
FEATURES             Location/Qualifiers
     source          1..3534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1091..1190)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1207..1226)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1208..1226)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1231..1247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1231..1247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1349..1368)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1360..2169
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1427..1446)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2316..2904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2805..2824
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3058..3075
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3234..3261)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3353..3439)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3488..3510
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3502..3519
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3503..3519
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggcatcgt cgtcgcggct ggatctcccg ccggggttcc gcttccaccc caccgacgag
      181 gaggtggtgt cgcactacct gacccacaag gcgctggaca gccgcttctc ctgcgtcgtc
      241 atcgccgacg ccgacctcaa caagatcgag ccgtgggatc tcccaagcaa ggctaagatg
      301 ggcgagaagg agtggttctt cttctgccac aaggaccgca agtacccgac ggggatgcgc
      361 accaaccgcg ccaccgccag cgggtactgg aaggccacgg ggaaggacaa ggagatcttc
      421 cgcggcagta gcagtagtcc ccgccgcgtg ctcgtcggca tgaagaagac gctcgtcttc
      481 tacacgggcc gcgcgccgcg cggaggcaag acgccgtggg tcatgcacga gtaccgcctc
      541 gagggcagcc tgccgcacaa cctccaccgc ggggccaagg acgaatgggc tgtgtgtaag
      601 gtgatcaaca aagacttggc gggcaaggct gggcaacaac aaatggcgcc gccgccgcac
      661 gccgtgtccg tgggcatgga gcgcagcgac tcgctggcct tcctcgacga cctggtgctc
      721 gacaacgccg acgacctgcc cccgctcgtc gactcgacaa cgtacgccgc cggctccctc
      781 ttcgccgcgg cggggacgac cacgacgacg aacgacgaca gcggcgggta ccaacaagcc
      841 accaaggcgg agccgcagcc gcagctgccc gcgccgagca acagcccgta ccagcaccag
      901 cagcaggcca tacggaggca ctgcaaggcg gaggcgccgg cgccggcgat ggtgctgagc
      961 ccgtcgcgcg agacgccggg cgcggacatg ttccagctcc agcatgtgga cgagctgctc
     1021 cagctggacg gcggcttcat ggaggactac tacaacatga acatgtggaa ggtcaagggt
     1081 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1141 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1201 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1261 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1321 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1381 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1441 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1501 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1561 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1621 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     1681 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1741 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     1801 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     1861 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     1921 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     1981 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2041 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2101 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2161 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2221 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2281 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2341 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2401 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2461 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2521 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2581 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2641 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2701 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2761 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2821 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2881 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2941 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3001 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3061 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3121 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3181 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3241 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3301 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3361 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3421 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3481 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
