LOCUS       sequence-130683-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces lentivirus expressing human EWSR1P552L mutant with FLAG tag
            at its N-terminus and Myc tag at its C-terminus by co-transfection 
            with psPAX2 and pMD2G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pSLIK-NFLAG-hEWSR1P552L-CMyc
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    140..164
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(191..220)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gacgctcgct gcgcccttcg tctgacgtgg cagcgctcgc cgtgaggagg ggggcgcccg
       61 cgggaggcgc caaaacccgg cgcggaggcc agatcttggg tgggttaatt aaccatcttt
      121 atggctcgag atgttatcaa ccactttgta caagaaagct gggtctagat atctcgagtg
      181 cggccgccta cagatcctct tctgagatga gtttttgttc gtagggccga tctctgcgct
      241 cctgacggtg ctcgccttta tccatttttc caggtcctcc acgtcctcct cttcttcctc
      301 ccatctgttc catcaaaggt ccagggggcc ccccagggcc acctcgtctt cctccaccaa
      361 agccacctcg gtccatgccc cggccaccac ggaagccacc tctgtctcca ccacggccac
      421 ctctgaacat tccaccggga ccaccacgat ccatgaggcc acctcttcct ccccgcatgc
      481 caccagggcc acctctgcca cgatcaccac ccgggggcgg aaagggtggc aggaggaagc
      541 cttcaggctt tggggcctta cactggttgc actctgttct ccaggcgaag ttctggtttc
      601 cacaacccgg attgggacac tgccagtctc cagctcggtg ctggacgttt cctcctccag
      661 aggggttccc tcgggaaccc cggggtcctc ttggagggaa gcctcctcta tctcctccac
      721 ggcctcccat gcgacc
//
