LOCUS       sequence-130684-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces lentivirus expressing human EWSR1 wild type with FLAG tag 
            at its N-terminus and Myc tag at its C-terminus by co-transfection 
            with psPAX2 and pMD2G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pSLIK-NFLAG-hEWSR1WT-CMyc
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    142..166
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(193..222)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aggacgctcg ctgcgccctt cgtctgacgt ggcagcgctc gccgtgagga ggggggcgcc
       61 cgcgggaggc gccaaaaccc ggcgcggagg ccagatcttg ggtgggttaa ttaaccatct
      121 ttatggctcg agatgttatc aaccactttg tacaagaaag ctgggtctag atatctcgag
      181 tgcggccgcc tacagatcct cttctgagat gagtttttgt tcgtagggcc gatctctgcg
      241 ctcctgacgg tgctcgcctt tatccatttt tccaggtcct ccacgtcctc ctcttcttcc
      301 tcccatctgt tccatcaaag gtccaggggg ccccccaggg ccacctcgtc ttcctccacc
      361 aaagccacct cggtccatgc cccggccacc acggaagcca cctctgtctc caccacggcc
      421 acctctgaac attccaccgg gaccaccacg atccatgagg ccacctcttc ctccccgcat
      481 gccaccaggg ccacctctgc cacgatcacc acccgggggc ggaaagggtg gcgggaggaa
      541 gccttcaggc tttggggcct tacactggtt gcactctgtt ctccaggcga agttctggtt
      601 tccacaaccc ggattgggac actgccagtc tccagctcgg tgctggacgt ttcctcctcc
      661 agaggggttc cctcgggaac cccggggtcc tcttggaggg aagcctcctc tatctcctcc
      721 acggcctccc atgcgaccca tgggtccc
//
