LOCUS       sequence-130693-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of ultrasensitive protein calcium sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, 
            Schreiter ER, Kerr RA, Orger MB, Jayaraman V, Looger LL, Svoboda K, 
            Kim DS
  TITLE     Ultrasensitive fluorescent proteins for imaging neuronal activity.
  JOURNAL   Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.
  PUBMED    23868258
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2013-07-18- 18"; volume: "499"; issue: "7458"; 
            pages: "295-300"
COMMENT     Sequence Label: pGP-CMV-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..105
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(109..117,118..132)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             160..216
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     429..450
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(556..577)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(817..836)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccgtcagatc cgctagcgct accggactca gatctcgcca ccatgggttc tcatcatcat
       61 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
      121 gatgacgata aggatctcgc caccatggtc gactcatcac gtcgtaagtg gaataagaca
      181 ggtcacgcag tcagagctat aggtcggctg agctcactcg agaacgtcta tatcaaggcc
      241 gacaagcaga agaacggcat caaggcgaac ttccacatcc gccacaacat cgaggacggc
      301 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      361 ctgcccgaca accactacct gagcgtgcag tccaaacttt cgaaagaccc caacgagaag
      421 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      481 gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc
      541 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      601 tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      661 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
      721 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      781 gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      841 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      901 ttcaaggagg acggcaacat cctgg
//
