LOCUS       sequence-130696-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Prickett TD, Wei X, Cardenas-Navia I, Teer JK, Lin JC, Walia V, 
            Gartner J, Jiang J, Cherukuri PF, Molinolo A, Davies MA, Gershenwald
            JE, Stemke-Hale K, Rosenberg SA, Margulies EH, Samuels Y
  TITLE     Exon capture analysis of G protein-coupled receptors identifies 
            activating mutations in GRM3 in melanoma.
  JOURNAL   Nat Genet. 2011 Sep 25;43(11):1119-26. doi: 10.1038/ng.950.
  PUBMED    21946352
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng"; 
            journalName: "Nat Genet"; date: "2011-09-25- 25"; volume: "43"; 
            issue: "11"; pages: "1119-26"
COMMENT     Sequence Label: pCDF1-GRM3 (E767K)
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             441..464
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        509..720
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             733..1001
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
ORIGIN
        1 aagattccag catgttgatc tctcttacct acgatgtgat cctggtgatc ttatgcactg
       61 tgtacgcctt caaaacgcgg aagtgcccag aaaatttcaa caaagctaag ttcataggtt
      121 ttaccatgta caccacgtgc atcatctggt tggccttcct ccctatattt tatgtgacat
      181 caagtgacta cagagtgcag acgacaacca tgtgcatctc tgtcagcctg agtggctttg
      241 tggtcttggg ctgtttgttt gcacccaagg ttcacatcat cctgtttcaa ccccagaaga
      301 atgttgtcac acacagactg cacctcaaca ggttcagtgt cagtggaact gggaccacat
      361 actctcagtc ctctgcaagc acgtatgtgc caacggtgtg caatgggcgg gaagtcctcg
      421 actccaccac ctcatctctg gattacaagg atgacgacga taagtaggcg gccgcgaagg
      481 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
      541 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
      601 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
      661 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
      721 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
      781 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
      841 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
      901 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
      961 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accgg
//
