LOCUS       sequence-130704-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD282
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(499..804)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(601..620)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ccttgagctc gatacggttg acgagggtgt ctccctcgaa cttgacctcg gcacgggtct
       61 tgtagtttcc gtcgtcctga aaatttaaat aatcagtagt tagtatatat atgtttaaac
      121 ttaccttgaa gaagatggta cgctcttgga cgtatccctc tggcatggcg gacttgaaga
      181 agtcgtgacg cttcatgtgg tctgggtaac gggagaagca ttggactccg tagcagaagg
      241 tggtgacgag ggttggccat gggactggga gctttccggt ggtgcagatg aacttgaggg
      301 tgagctttcc gtaggtggcg tctccctctc cctctccgga gacggagaac ttgtgtccgt
      361 tgacgtctcc gtcgagctcg acgaggattg ggacaactcc agtgaacaat tcttctcctt
      421 tactcatcga tgctcctgag gctcccgatg ctccctaggt cgacctgcag actggctgtg
      481 tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
      541 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
      601 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
      661 acgggggact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
      721 gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
      781 ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcggca aa
//
