LOCUS       sequence-130705-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD282
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             481..501
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(519..552)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             562..591
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tagttcgagg gagacaccct cgtcaaccgt atcgagctca aggtaagttt aaacagttcg
       61 gtactaacta accatacata tttaaatttt cagggaatcg acttcaagga ggacggaaac
      121 atcctcggac acaagctcga gtacaactac aactcccaca acgtctacat catggccgac
      181 aagcaaaaga acggaatcaa ggtcaacttc aaggtaagtt taaacatgat tttactaact
      241 aactaatctg atttaaattt tcagatccgt cacaacatcg aggacggatc cgtccaactc
      301 gccgaccact accaacaaaa caccccaatc ggagacggac cagtcctcct cccagacaac
      361 cactacctct ccacccaatc cgccctctcc aaggacccaa acgagaagcg tgaccacatg
      421 gtcctcctcg agttcgtcac cgccgccgga atcacccacg gaatggacga gctctacaag
      481 gagaatctgt actttcaatc cggaaaggta agtttaaaat aacttcgtat agcatacatt
      541 atacgaagtt attttcaggg agagcaaaag cttatctccg aggaggacct ctaaggatga
      601 tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt ttttttccta
      661 ttatttattt attctctttg tgtttttttt cttgctttct aaaaaattaa ttcaatccaa
      721 atctaaacat ttttttttct ctttccgtct cccaattcgt attccgctcc tctcatctga
      781 acacaatgtg caagtttatt tatcttctcg ctttcatttc attaggacgt gg
//
