LOCUS       sequence-130714-        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 PC
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    174..198
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctgcagggta ccacgcgtac ccaacacacg gacacatgca cgaacacgca tgcagagaga
       61 tcatacatca tctatagaga agtacacgta aaagtaagac tcgtcgtctg ccagctacaa
      121 ccgagaaggc gacgtgaaag gaacaagaaa ggaggaggag ggcggccgct tacaccactt
      181 tgtacaagaa agttgggcac tcgatctcca ggatgaggtc gtcaccttcc agtgtcatgt
      241 ccttggtcac atgaaccttg cggacagtac cctccatggg tgaggtcacc acagtctcca
      301 tcttcatggc actgagcaca cacaggggct ggcccttggc caccttggcc cctgccacca
      361 ctttgatgtc tatcaccttc ccaggcatgg gcgccccgat ctggcccttc acgtccttta
      421 gggccttggg gtggaagtgc atctccttca tggcctgggt gtccttgacc aagatggacc
      481 gcagctgccc attgagctca aagaagacct gcctctggcc ggcccggttc aggtcgctca
      541 cggccagggc tttgatgtgc agcgtcttgc cccgctccag ctccacctca aactcctctg
      601 cgatcttggg tccctgcagg aagaggcgag tattcaggct atccaggggg ccaaaggtgg
      661 cagtgaagtc cttgaagtgg gcaaacacat cggggtacat agctgctgag agcacatctt
      721 ccggcgtcac ctcctcccca tgccggtcta ccagctcctt ctccagtgcc tgcagatcca
      781 ggggagggag ggaggctcca ggccgcccct ccacccttgg caggtccttc agtaccttag
      841 agcgaaaggg ttcggggaac cccccatggg ggacaccgat gtagccctgc aggaactcca
      901 ccacggagcg gggaaaggac agctcttccg cctgagcttc ggcctctgcc cggctcaatc
      961 cattctgcac cataaactgg gccaggtccc ccacgatctt ggaggagggc gtcaccttga
     1021 tgagatcgcc cagcatctgg ttggcctcca cataggcctt cttgacctcc ttgaacttgg
     1081 agccaagccc catgctgtgg gcctg
//
