LOCUS       sequence-130720-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 TP53
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    177..201
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttctgcagg gtaccacgcg tacccaacac acggacacat gcacgaacac gcatgcagag
       61 agatcataca tcatctatag agaagtacac gtaaaagtaa gactcgtcgt ctgccagcta
      121 caaccgagaa ggcgacgtga aaggaacaag aaaggaggag gagggcggcc gcttacacca
      181 ctttgtacaa gaaagttggg ctgtctgagt caggcccttc tgtcttgaac atgagttttt
      241 tatggcggga ggtagactga ccctttttgg acttcaggtg gctggagtga gccctgctcc
      301 cccctggctc cttcccagcc tgggcatcct tgagttccaa ggcctcattc agctctcgga
      361 acatctcgaa gcgctcacgc ccacggatct gaagggtgaa atattctcca tccagtggtt
      421 tcttctttgg ctggggagag gagctggtgt tgttgggcag tgctcgctta gtgctccctg
      481 ggggcagctc gtggtgaggc tcccctttct tgcggagatt ctcttcctct gtgcgccggt
      541 ctctcccagc acaggcacaa acacgcacct caaagctgtt ccgtcccagt agattaccac
      601 tggagtcttc cagtgtgatg atggtgagga tgggcctccg gttcatgccg cccatgcagg
      661 aactgttaca catgtagttg tagtggatgg tggtacagtc agagccaacc tcaggcggct
      721 catagggcac caccacacta tgtcgaaaag tgtttctgtc atccaaatac tccacacgca
      781 aatttccttc cactcggata agatgctgag gaggggccag accatcgcta tctgagcagc
      841 gctcatggtg ggggcagcgc ctcacaacct ccgtcatgtg ctgtgactgc ttgtagatgg
      901 ccatggcgcg gacgcgggtg ccgggcgggg gtgtggaatc aacccacagc tgcacagggc
      961 aggtcttggc cagttggcaa aacatcttgt tgagggcagg ggagtacgtg caagtcacag
     1021 acttggctgt cccagaatgc aagaagccca gacggaa
//
