LOCUS       sequence-130727-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for cell-free expression with N-term 
            8xHis eGFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Gagoski D, Mureev S, Giles N, Johnston W, Dahmer-Heath M, Skalamera 
            D, Gonda TJ, Alexandrov K
  TITLE     Gateway-compatible vectors for high-throughput protein expression in
            pro- and eukaryotic cell-free systems.
  JOURNAL   J Biotechnol. 2015 Feb 10;195:1-7. doi: 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19.
  PUBMED    25529348
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2015-02-10"; volume: "195:1-7. doi"; pages: " 
            10.1016/j.jbiotec.2014.12.006. Epub 2014 Dec 19"
COMMENT     Sequence Label: pCellFree_G03
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    178..302
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        445..547
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(614..633)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cttcctgcag ggtaccacgc gtacccaaca cacggacaca tgcacgaaca cgcatgcaga
       61 gagatcatac atcatctata gagaagtaca cgtaaaagta agactcgtcg tctgccagct
      121 acaaccgaga aggcgacgtg aaaggaacaa gaaaggagga ggagggcggc cgcttacacc
      181 actttgtaca agaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
      241 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
      301 tgaagctaag ttggcagcat cacccgacgc actttgcgcc gaataaatac ctgtgacgga
      361 agatcacttc gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc
      421 caacttttgg cgaaaatgag acgttgatcg gcacgcaaga ggttccaact ttcaccataa
      481 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
      541 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
      601 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
      661 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
      721 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
      781 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
      841 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
      901 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
      961 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     1021 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     1081 gcaaggc
//
