LOCUS       sequence-13074-e        3651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1179-HQ858720
FEATURES             Location/Qualifiers
     source          1..3651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1208..1307)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1324..1343)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1325..1343)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1348..1364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1348..1364)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1466..1485)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1477..2286
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1544..1563)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2433..3021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2922..2941
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3175..3192
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3351..3378)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3470..3556)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3605..3627
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3619..3636
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3620..3636
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggcgtcga cctctccgcg ccgctcctct ggcggggact cagcttcccc gtccgcaccc
      181 ctcatcccct cgccgccgac ctccccggca tccggcccct ccggtggccc gctagggcgg
      241 ctcacgagcc tccgcggcgc ggcgcggttc atccgccgca cggggagccg gcgcctgatg
      301 agggagccct ccgtggcggt gcgggagacg gccgccgagc acctcgagga gcgccagacc
      361 gactgggcct actcgaagcc cgtcgtcgtg ctcgacatgc tctggaacct cgccttcgtc
      421 gccgtcgccg cggccgtact cgccgcatcg gtgggcgagc ggcccaccgt gccgctccgt
      481 gtctggatcg taggctacgt gctccagtgc ctcctccacg tactcagcgt caccgtcgag
      541 tacaggcgtc gacgcaggaa cgcggaccag gagggagcgg gtgatgagga tttaaagctc
      601 agtattgtga agcacttgga gtctgcaaac acgatgtttt ccttcatatg gtggattgtt
      661 gggttctatt gggtgtctgc aggtggtaaa gctttatcac atgatgctcc tcagctttat
      721 tggctgtcaa ttgtttttct ggcgtttgat gttttctttg tggtcttctg tgttgccttg
      781 gcttgtgtga ttggaatcgc tgtttgttgc tgccttccct gcattattgc tatcttatat
      841 gctgtaactg atcagcagga gggagcatct gaagatgaca taaacaacct atccaaattc
      901 aaatttcaga caatgagtga tgcagacaag ctagctgctg gcattgcagc acctgtgggt
      961 ggagtgatga cagagtgtgg taccaatcct cctgtcgaac atattctctc agctgaggat
     1021 gcagagtgct gtatctgcct atgcccgtat gaagatggcg tggaactacg tgagcttcct
     1081 tgcaaccacc attttcactg cagctgcatt gacaagtggc ttcacataaa tgctacatgc
     1141 ccattgtgca agttcgacat catcaagagc aaccgtgaca tagaagaggt caagggtggg
     1201 cgcgccgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1261 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     1321 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1381 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1441 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1501 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1561 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1621 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1681 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1741 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     1801 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     1861 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     1921 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     1981 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2041 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2101 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2161 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2221 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2281 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2341 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2401 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2461 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2521 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     2581 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2641 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2701 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2761 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2821 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2881 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2941 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3001 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3061 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3121 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3181 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3241 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3301 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3361 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3421 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3481 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3541 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     3601 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
