LOCUS       sequence-130747-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_COF1H02
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(560..659)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(676..695)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(677..695)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(700..716)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(700..716)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc ttcatggcca gcgaggaact atttgaggct
      121 gaattctcgg aagaacagga cttggagcat cagcaggcca tggagactga ggaggcggag
      181 ctagcggaaa cagaagagcc tttggaaatc accgaggaat cgccggataa tcctgaggca
      241 gaatctacaa cagaacaatt gacagaaaaa ccagtgacga atggcaacaa ggcccctgct
      301 gataacgaag ccaagatgac acaactgccg ttggccagga tacggaacat aatgaaacta
      361 gatccggatc tgcatatggc caacaatgag gccgtgttca tagtggcgaa ggcagtggaa
      421 ctattcatag cctcgttgtc acgggaatca tatacctaca cggcgcagtc caaaaagaaa
      481 accatacaaa agagggacgt ggatatggcc atttctgctg tggacagttt gctattcctt
      541 gatggagcca tgaactttga cccagctttc ttgtacaaag ttggcattat aagaaagcat
      601 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
      661 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
      721 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaa
//
