LOCUS       sequence-130748-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_COF1C03
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(581..680)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(697..716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(698..716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(721..737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(721..737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(839..858)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(917..936)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gatttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc ttcatgacta gctttcggga aatgcgtttc
      121 gatgatttgt tcaaaattaa ttcactagtt ttcgatgctc tcaccgaggt ttatagcctg
      181 accttttttg taaagcattt tttggagttt ccaggactct cacagattgc catagctcca
      241 ggtcctgatg gcagacccat gggatatata ttcgggcagt atcaggtcaa gcgcaaccag
      301 gatccctatg gtcatgtggc agcattgact gtatctccgg agtaccgacg ccttggactg
      361 gccactgctc taatggattt tttctttatg gtctcagacc ttaaaggagc ttcctatgtt
      421 aacctcttta tgcgaatcag taatcgggct gcctatcaat tgtacacttc tttgggttat
      481 gctcatcgcc aaacattcct ggactactat ccggatgaac cgaagccaga aagtgcctac
      541 gaattgagaa agtacgttcc ccgccccatg gaggttcaag acccagcttt cttgtacaaa
      601 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
      661 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
      721 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
      781 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
      841 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
      901 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
      961 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt tt
//
