LOCUS       sequence-130768-        2108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag
            at its N-terminus and Myc tag at its C-terminus by co-transfection 
            with psPAX2 and pMD2G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pSLIK-NFLAG-hEWSR1G511A-CMyc
FEATURES             Location/Qualifiers
     source          1..2108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..75
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2041..2070
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 twaagacacc gggaccgatc cagcctccgc ggccccgaac tagtcaccat ggactacaag
       61 gacgacgatg acaaaatggc gtccacggat tacagtacct atagccaagc tgcagcgcag
      121 cagggctaca gtgcttacac cgcccagccc actcaaggat atgcacagac cacccaggca
      181 tatgggcaac aaagctatgg aacctatgga cagcccactg atgtcagcta tacccaggct
      241 cagaccactg caacctatgg gcagaccgcc tatgcaactt cttatggaca gcctcccact
      301 ggttatacta ctccaactgc cccccaggca tacagccagc ctgtccaggg gtatggcact
      361 ggtgcttatg ataccaccac tgctacagtc accaccaccc aggcctccta tgcagctcag
      421 tctgcatatg gcactcagcc tgcttatcca gcctatgggc agcagccagc agccactgca
      481 cctacaagac cgcaggatgg aaacaagccc actgagacta gtcaacctca atctagcaca
      541 gggggttaca accagcccag cctaggatat ggacagagta actacagtta tccccaggta
      601 cctgggagct accccatgca gccagtcact gcacctccat cctaccctcc taccagctat
      661 tcctctacac agccgactag ttatgatcag agcagttact ctcagcagaa cacctatggg
      721 caaccgagca gctatggaca gcagagtagc tatggtcaac aaagcagcta tgggcagcag
      781 cctcccacta gttacccacc ccaaactgga tcctacagcc aagctccaag tcaatatagc
      841 caacagagca gcagctacgg gcagcagagt tcattccgac aggaccaccc cagtagcatg
      901 ggtgtttatg ggcaggagtc tggaggattt tccggaccag gagagaaccg gagcatgagt
      961 ggccctgata accggggcag gggaagaggg ggatttgatc gtggaggcat gagcagaggt
     1021 gggcggggag gaggacgcgg tggaatgggc gctggagagc gaggtggctt caataagcct
     1081 ggtggaccca tggatgaagg accagatctt gatctaggcc cacctgtaga tccagatgaa
     1141 gactctgaca acagtgcaat ttatgtacaa ggattaaatg acagtgtgac tctagatgat
     1201 ctggcagact tctttaagca gtgtggggtt gttaagatga acaagagaac tgggcaaccc
     1261 atgatccaca tctacctgga caaggaaaca ggaaagccca aaggcgatgc cacagtgtcc
     1321 tatgaagacc cacccactgc caaggctgcc gtggaatggt ttgatgggaa agattttcaa
     1381 gggagcaaac ttaaagtctc ccttgctcgg aagaagcctc caatgaacag tatgcggggt
     1441 ggtctgccac cccgtgaggg cagaggcatg ccaccaccac tccgtggagg tccaggaggc
     1501 ccaggaggtc ctgggggacc catgggtcgc atgggaggcc gtggaggaga tagaggaggc
     1561 ttccctccaa gaggaccccg gggttcccga gggaacccct ctggagcagg aaacgtccag
     1621 caccgagctg gagactggca gtgtcccaat ccgggttgtg gaaaccagaa cttcgcctgg
     1681 agaacagagt gcaaccagtg taaggcccca aagcctgaag gcttcctccc gccacccttt
     1741 ccgcccccgg gtggtgatcg tggcagaggt ggccctggtg gcatgcgggg aggaagaggt
     1801 ggcctcatgg atcgtggtgg tcccggtgga atgttcagag gtggccgtgg tggagacaga
     1861 ggtggcttcc gtggtggccg gggcatggac cgaggtggct ttggtggagg aagacgaggt
     1921 ggccctgggg ggccccctgg acctttgatg gaacagatgg gaggaagaag aggaggacgt
     1981 ggaggacctg gaaaaatgga taaaggcgag caccgtcagg agcgcagaga tcggccctac
     2041 gaacaaaaac tcatctcaga agaggatctg taggcggccg cactcgagat atctagaccc
     2101 agctttta
//
