LOCUS       sequence-130777-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the membrane targeted Y-GECO1 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, 
            Campbell RE, Harrison DJ
  TITLE     Microfluidic cell sorter-aided directed evolution of a protein-based
            calcium ion indicator with an inverted fluorescent response.
  JOURNAL   Integr Biol (Camb). 2014 May 19.
  PUBMED    24840546
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Integr Biol
            (Camb). 2014 May 19."
COMMENT     Sequence Label: AAV-hSyn-Lyn-Y-GECO1
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(262..276)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agctttcact ttgctgtcat catttgtaca
       61 aactcttcgt agtttacctg accatccccg tcggagtctg ctaccctgat catttcatca
      121 aactcttcat ctgttaactt ctctccaagg tttgtcatca cgtggcgaag ctctgctgcg
      181 ccgatgtagc cattgccgtc cttatcaaac acacggaacg cttctctaat ttcctcttca
      241 ctgtccgtgt cattcatttt tcttgtcatc atcgtcagga actcagggaa gccgattgtg
      301 ccgtcaccgt cggcatctac ttcattgatc atgtcccgca gctctgcttc tgaggggttc
      361 tgccccagag accgcagcac cgtccccagc tcctcggttg tcatcgtccc atccccgtcc
      421 ttgtcaaata gggagaaagc ctctttaaat tctgcgatct gctcttcagt cagttggtca
      481 cgcgtgtcag cttcccaatt agtggtcatt ttcttcatta cggggccgtc ggcgggaaaa
      541 ttcaccccat agaagatact tttgtgatag atgcagttct ccttttcaga gactgttatg
      601 tccgcgttgc atcgacagac cgctccgtcc tcatagaaga agctcctttc ccacgtatag
      661 ccctctggac atgagttctt gaagtaatct acaatatcct gtgggtactc tgtgaa
//
