LOCUS       sequence-13078-e        3999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1392-HQ858724
FEATURES             Location/Qualifiers
     source          1..3999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1556..1655)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1672..1691)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1673..1691)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1696..1712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1696..1712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1814..1833)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1825..2634
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1892..1911)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2781..3369
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3270..3289
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3523..3540
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3699..3726)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3818..3904)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3953..3975
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3967..3984
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3968..3984
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgcgcgtt caaaatgctt caccggaggt gtcttcaagg
      181 gaccacatac aaaaaaaact tcaaacggtc ctgttttttt ttcccaaaaa cttttacaat
      241 ttccgttcca taatggcctt ggtcgtatgc agctgtttat ataaaatgcc taaaccccgt
      301 acccctagtc cccgaccacc tcaccgcatc gccaccggcc caccgcgcag ccagatggcc
      361 tcttcctcct cgtgcccgct cgcccccggg acaagcggat gcaagcccag gacgacgcgg
      421 accagcggcg tcgggttcgg agtcgaagca atggcgcgga gcgtggcggc agtgaagctg
      481 gagccgaagc cggagccgtg ggcggcggaa gcagaggagg ggtcgtcagt tgtgccgcgg
      541 tcgatggagg ggccaccgtt tccggcgccg ttcgtggcga agacgtacga gatggtgtcg
      601 gacgcggcca cggacgccgt agtgtcgtgg gcgccctgcg gcgcggggaa cactttcgtg
      661 gtgtgggacc cgcaggcgct cgccaccggc atcctcccgc gcttcttcaa gcacgccaac
      721 ttcgcgtcct tcgtcaggca gctcaacgtc tacggattca ggaaggtgaa tccagatcgt
      781 tgggagtttg caaacgaatc attcttagca ggccagaagc atctgctgaa aagcatcagg
      841 aggaggcgcg cgtccaagcc tcaggtagaa gcatcgccaa gaaacagcgc aagtgcttgt
      901 tccggacagc ccaacaagga tcccggggtg gtcgagagtt tgaaacggga ccgcgcagcg
      961 ctcagggctg aagtcatcac gctaaggcag cagtacagca tttgcaaatc ccaactcgtc
     1021 gccctggagg agaggatcct gaacaacgag agggaccagc agaaggccat cgccttcttc
     1081 gcaaaggtgc tcagcaaccc agctttcgtg cagcaggtcc tgcgcaacta cgccagggag
     1141 agagagctac acggcgcttc caaaagacag cggctgatgg aaaacgaagg acatcggcag
     1201 ggcgatttgc cactgagggg tagcaccgaa gccgcgtttg cgaccggtgc ggctggcgtc
     1261 tctgtgggga ctgggagcac aaatgttggc acagccgcga gacaggagga gtctggttct
     1321 gagttgaatg atcaggaggt gaacagtatt tggtctgacg tgtgggacga gctggacctg
     1381 gacctggacg cggtaccagg cgctgaaatg gaccggcgag aagcggacaa gggcgtcgct
     1441 ggcttccacg acgtcgagga cttgagtggc cggccttgcg gttggggtga tgagttctcg
     1501 tatctcgcgg agccgatgca gttcgtggag cactatgaca agggtgggcg cgccgaccca
     1561 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1621 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1681 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1741 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1801 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1861 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1921 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1981 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2041 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     2101 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2161 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2221 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2281 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2341 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     2401 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2461 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2521 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2581 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     2641 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     2701 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     2761 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2821 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2881 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2941 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3001 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3061 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3121 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     3181 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3241 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3301 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3361 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3421 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3481 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3541 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3601 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3661 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     3721 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     3781 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     3841 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     3901 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     3961 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
