LOCUS       sequence-130784-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_COF1G01
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(477..576)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(593..612)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(594..612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(617..633)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(617..633)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg cttcatgatg tcgtcggatc aacagttctt
      121 tctaaaatgg aacgattttc aaacgaatat ggtgacctcg ttccgtcacc tgcgcgacga
      181 gaagagcttc acagatgtaa cactcgcctg cgagggccaa acctgcaaag cccacaaaat
      241 ggtgctttcc gcttgcagtc cctactttaa agcgctactg gaggagaacc catcgaagca
      301 tccgatcatt atcctgaaag atgtctccta cattcaccta caggctatac tggagttcat
      361 gtacgccggt gaggtgaacg tgtcccagga acaattgcca gcatttctta agaccgccga
      421 tcgcctcaaa gtgaaaggcc tcgcagagac acccagttcg ataaagcggg aaggtgaccc
      481 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
      541 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
      601 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
      661 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
      721 cataaacagt aatac
//
