LOCUS       sequence-130795-        1266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Y-GECO1 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, 
            Campbell RE, Harrison DJ
  TITLE     Microfluidic cell sorter-aided directed evolution of a protein-based
            calcium ion indicator with an inverted fluorescent response.
  JOURNAL   Integr Biol (Camb). 2014 May 19.
  PUBMED    24840546
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Integr Biol
            (Camb). 2014 May 19."
COMMENT     Sequence Label: AAV-Y-GECO1
FEATURES             Location/Qualifiers
     source          1..1266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             1026..1040
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 atggtcgact catcacgtcg taagtggaat aagacaggtc acgcagtcag agctataggc
       61 cggctgagct cactcgaggg ttatagtacc gagaaaattg tgcctgttcc aaagcaaggg
      121 atattaaaag gaaaggtcag catgtaccta ttgctgaagg atggcggtcg ttatcattgc
      181 cagtttgata cggtatataa agctaaatca gtgccctcca agatgccagg acggcatttt
      241 atacagcata agctccttag ggaggaccgc tctgatgcta aaaaccataa atggcaatta
      301 acagaacatg caatcgcagg catggacgag ctgtacaagg gaggtacagg cgggagtatg
      361 gtgagcaagg gcgaggggca atcgaagcat gggctaaagg aggagatgac ggtgaagtac
      421 cacatggaag gttgcgttaa tggtcacaaa tttgtcatta ctggagaggg cattggaaac
      481 ccttttaagg gtaagcaaac tgcaaatttg tgtgtgatag aaggaggccc gctgccgttc
      541 tcggaggaca ttttaagtcc gggttttaaa tatggtgacc ggattttcac agagtaccca
      601 caggatattg tagattactt caagaactca tgtccagagg gctatacgtg ggaaaggagc
      661 ttcttctatg aggacggagc ggtctgtcga tgcaacgcgg acataacagt ctctgaaaag
      721 gagaactgca tctatcacaa aagtatcttc tatggggtga attttcccgc cgacggcccc
      781 gtaatgaaga aaatgaccac taattgggaa gctgacacgc gtgaccaact gactgaagag
      841 cagatcgcag aatttaaaga ggctttctcc ctatttgaca aggacgggga tgggacgatg
      901 acaaccgagg agctggggac ggtgctgcgg tctctggggc agaacccctc agaagcagag
      961 ctgcgggaca tgatcaatga agtagatgcc gacggtgacg gcacaatcgg cttccctgag
     1021 ttcctgacga tgatgacaag aaaaatgaat gacacggaca gtgaagagga aattagagaa
     1081 gcgttccgtg tgtttgataa ggacggcaat ggctacatcg gcgcagcaga gcttcgccac
     1141 gtgatgacaa accttggaga gaagttaaca gatgaagagt ttgatgaaat gatcagggta
     1201 gcagactccg acggggatgg tcaggtaaac tacgaagagt ttgtacaaat gatgacagca
     1261 aagtga
//
