LOCUS       sequence-130806-         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Shen S, Rodrigo G, Prakash S, Majer E, Landrain TE, Kirov B, Daros 
            JA, Jaramillo A
  TITLE     Dynamic signal processing by ribozyme-mediated RNA circuits to 
            control gene expression.
  JOURNAL   Nucleic Acids Res. 2015 May 26;43(10):5158-70. doi: 
            10.1093/nar/gkv287. Epub 2015 Apr 27.
  PUBMED    25916845
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv287"; journalName: "Nucleic Acids Res"; date: 
            "2015-05-26- 26"; volume: "43"; issue: "10"; pages: "5158-70"
COMMENT     Sequence Label: pSCKtheoRaj12
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    56..77
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    282..300
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    307..325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    370..386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             433..444
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt
       61 cgcggccgct tctagagaca gaaaagcccg cctttcggcg ggctttgccc agaatccctt
      121 cgagcaaatc ctagcgaggt gccgctagat tcgctcaaag gaacccttct tcctgcccgt
      181 ctggaatcca ggacacctgc caagggcctt tcggctggta tgtatttcgt cctatttggg
      241 actcatcagc tggatgtacg aaggcagcgc cgtgctcagt atctctatca ctgataggga
      301 tgtcaatctc tatcactgat agggagctag ccaattgatg catataaatg tgagcggata
      361 acattgacat tgtgagcgga taacaagata ctgagcacac ccagtatcat tctcttcttc
      421 ctgcccacgc ggaaagagga gaaaggtgta atgactagtc gtaaaggcga agagctgttc
      481 actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc
      541 gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt
      601 actactggta
//
