LOCUS       sequence-130809-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD283
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             234..539
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     418..437
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(587..610)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(614..637)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(641..664)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    683..716
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
       61 tcaaggcata tatgatgtca atatctccgg ttcggtaagc acaaccatgc agaatgaagc
      121 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
      181 ccggtttatt gaaatgaacg gctcttttgc cgacgagaac aggggctggt gaaatgcagt
      241 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
      301 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
      361 cagataaagt cccccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
      421 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
      481 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
      541 tgtcaggctc ccttatacac agccagtctg caggtcgaac tagtctcttg tcatcgtcat
      601 ccttgtaatc acgcttgtcg tcgtcgtcct tgtagtcacg cttatcgtca tcgtctttat
      661 aatcagatcc ggctccctga aaataacttc gtataatgta tgctatacga agttattaaa
      721 aattgcggtc ataaactgaa acgtaacata tgataaggta ttttgtgtgc ggttttttct
      781 atgatgtttc aaaa
//
