LOCUS       sequence-13081-e        3306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1395-HQ858727
FEATURES             Location/Qualifiers
     source          1..3306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(863..962)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(979..998)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(980..998)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1003..1019)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1003..1019)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1121..1140)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1132..1941
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1199..1218)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2088..2676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2577..2596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2830..2847
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3006..3033)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3125..3211)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3260..3282
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3274..3291
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3275..3291
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggcggcg gcgaacggag ctgccggtgc ggtgctgttc
      181 agcgaggagg acctgaggga ggtgagtggg gtgcgctggg gagaggactt cgtggaggtg
      241 acgtgcggat gcaccagcca ccgctacggc gacgccgtcg gccgcctccg cgtctttgcg
      301 tcaggcgacc tggagatcaa ctgcgagtgc acgcccggct gccgcgaaga caagatgaca
      361 cctgctgcct ttgagaagca ctccggaaag gagacagctg gaaagtggag gaacaccgtc
      421 tgggtaatgg tccaggggga aaaagtgcca ctgtcaaaga cggctctgct caaatactac
      481 tacctggcgc acaagtcagg caacggttct cacaagggcc gtaacggacg tccgtcccac
      541 cgcgacgagt tcatctgctg cgcaggatgt ggcaaggaga ggaggtttcg gctccggtca
      601 aaggaagagt gccgtgttta ccatgacgct cttgccaaag ctagctggac gtgtgcggat
      661 ttgacaactg atagggtcac ctgtgacgac gaggaggagc gtgcaagccg caaggtcctg
      721 cggggctgct cccgcgcgcc gtcctgcacg ggctgcatga agtgcgtgtg cttcggctgc
      781 gagacctgcc gcttcaagga ctgcgactgc cagacctgcg ttgacttcta ccgcaactcc
      841 aaggaaaagg gtgggcgcgc cgacccagct ttcttgtaca aagttggcat tataagaaag
      901 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
      961 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1021 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1081 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1141 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1201 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1261 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1321 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1381 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     1441 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1501 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1561 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1621 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     1681 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     1741 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     1801 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     1861 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     1921 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     1981 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2041 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2101 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2161 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2221 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2281 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2341 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2401 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2461 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2521 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2581 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2641 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2701 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2761 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2821 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     2881 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     2941 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3001 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3061 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3121 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3181 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     3241 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     3301 gggccc
//
