LOCUS       sequence-130816-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD283
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             456..476
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(494..527)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             537..566
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 accgtatcga gctcaaggta agtttaaaca gttcggtact aactaaccat acatatttaa
       61 attttcaggg aatcgacttc aaggaggacg gaaacatcct cggacacaag cttgagtaca
      121 actacaactc ccacaacgtc tacatcaccg ccgacaagca aaagaacgga atcaaggcca
      181 acttcaaggt aagtttaaac atgattttac taactaacta atctgattta aattttcaga
      241 tccgtcacaa catcgaggac ggaggagtcc aactcgccga ccactaccaa caaaacaccc
      301 caatcggaga cggaccagtc ctcctcccag acaaccacta cctctcctac caatccaagc
      361 tcttcaagga cccaaacgag aagcgtgacc acatggtcct ccttgagttc ctcaccgccg
      421 ccggaatcac cgagggaatg aacgagctct acaaggagaa tctgtacttt caatccggaa
      481 aggtaagttt aaaataactt cgtatagcat acattatacg aagttatttt cagggagagc
      541 aaaagcttat ctccgaggag gacctctaag gatgatcgac gccaacgtcg ttgaattttc
      601 aaattttaaa tactgaatat ttgttttttt tcctattatt tatttattct ctttgtgttt
      661 tttttcttgc tttctaaaaa attaattcaa tccaaatcta aacatttttt tttctctttc
      721 cgtctcccaa ttcgtattcc gctcctctca tctgaacaca atgtgcaagt ttatttatct
      781 tctcgctttc atttcattag gacgtggggg gaattggtgg aa
//
