LOCUS       sequence-130820-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_COF1D03
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(589..688)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(705..724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(706..724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(729..745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(729..745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagcag gcttcatgtc gttgccacgc gtttacttcg
      121 atatagccgc aggaggtgag aagttaggta gaattgtgat ggaactacgt tcggatgtgg
      181 tgcccaagac ggcggagaat ttccgggccc tctgcaccgg agagaagggg tacggctaca
      241 agggatcacc attccaccgc gtcatcccga atttcatgtg tcagggcggt gatttcacta
      301 atcaaaacgg aactggagga cgctccattt atggaaacaa gtttcccgat gagaatttcg
      361 agctaaagca taccggagct ggtgtacttt cgatggccaa tgctggggcc aataccaatg
      421 gatctcagtt tttcatttgc accgggaaaa ccacatggct ggataataag catgtcgtgt
      481 tcggcaaggt cgttgaggga atggatatcg tccagaaggt agaatcttac ggttcacaag
      541 atgggaagac cagcaagaaa gtaattatcg aagactgcgg agccctcgac ccagctttct
      601 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      661 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
      721 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
      781 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
      841 gtaatacaag ggg
//
