LOCUS       sequence-130821-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD284
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             243..548
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     427..446
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(596..619)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(623..646)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(650..673)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    692..725
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgagtgtaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct
       61 atcagttgct caaggcatat atgatgtcaa tatctccggt tcggtaagca caaccatgca
      121 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
      181 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgcc gacgagaaca ggggctggtg
      241 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
      301 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
      361 gtctgctgtc agataaagtc ccccgtgaac tttacccggt ggtgcatatc ggggatgaaa
      421 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
      481 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
      541 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgaact agtctcttgt
      601 catcgtcatc cttgtaatca cgcttgtcgt cgtcgtcctt gtagtcacgc ttatcgtcat
      661 cgtctttata atcagatccg gctccctgaa aataacttcg tataatgtat gctatacgaa
      721 gttattaaaa attgcggtca taaactgaaa cgtaacatat gataaggtat tttgtgtgcg
      781 gttttttcta tgatgtttca aaaaaattta tcag
//
