LOCUS       sequence-130825-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD284
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             536..556
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(574..607)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             617..646
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgaggacgga ggagtcctca ccgccaccca agacacctcc ctccaagacg gatgcctcat
       61 ctacaacgtc aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
      121 tcagatccgt ggagtcaact tcccatccaa cggaccagtc atgcaaaaga agaccctcgg
      181 atgggaggcc aacaccgaga tgctctaccc agccgacgga ggactcgagg gacgtaccga
      241 catggccctc aagctcgtcg gaggaggaca cctcatctgc aacttcaaga ccacctaccg
      301 ttccaaggta agtttaaaca tgattttact aactaactaa tctgatttaa attttcagaa
      361 gccagccaag aacctcaaga tgccaggagt ctactacgtc gaccaccgtc tcgagcgtat
      421 caaggaggcc gacaaggaga cctacgtcga gcaacacgag gtcgccgtcg cccgttactg
      481 cgacctccca tccaagctcg gacacaagct caacggaatg gacgagctct acaaggagaa
      541 tctgtacttt caatccggaa aggtaagttt aaaataactt cgtatagcat acattatacg
      601 aagttatttt cagggagagc aaaagcttat ctccgaggag gacctctaag gatgatcgac
      661 gccaacgtcg ttgaattttc aaattttaaa tactgaatat ttgttttttt tcctattatt
      721 tatttattct ctttgtgttt tttttcttgc tttctaaaaa attaattcaa tccaaatcta
      781 aacatttttt tttctctttc cgtctcccaa ttcgtattcc gctcctctca tctgaacaca
      841 atgtgcaagt ttatttatct tctcgc
//
