LOCUS       sequence-13084-e        3339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1398-HQ858730
FEATURES             Location/Qualifiers
     source          1..3339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(896..995)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1012..1031)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1013..1031)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1036..1052)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1036..1052)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1154..1173)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1165..1974
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1232..1251)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2121..2709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2610..2629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2863..2880
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3039..3066)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3158..3244)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3293..3315
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3307..3324
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3308..3324
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgatgtcg tcggcgccgg aggagacgct gttcaacctg
      181 gaccagcagc agcctccgcc ggcggagcag gagcagatct gctacgtgca ctgcagctac
      241 tgcgacacca tcctcgcggt gggcgtgccg tgcagcagct tgttccagac agtcaccgtg
      301 cggtgcggcc actgctccaa cctgctctac gtcaacctcc gcgccctcct gctgccggcg
      361 gcggcggcca ataaccagct accacccttc ggccagcctc tcctctcccc cacctccccg
      421 cacggtctac ttgcgccgag cctgccgggt gctgaaccgc cgagcgcttg cgtgagcggc
      481 gtcacgagca tcaacaacac gtgcggtggc aacaccacca cgtcgtcggc catgtcgtcc
      541 atggcgccgc caccggccaa acatgcactg caggaggcgc agcagctgcc caggaccgca
      601 gcgtcggtga acagaacttc agagaagcgg cagagagttc cttccgcgta caaccgtttc
      661 atcaaagatg aaatccagcg catcaaggcc agcaatccgg acatcactca cagggaagct
      721 ttcagcgcgg ctgccaagaa ttgggcccat ttcccccaca tccatttcgg cctcatgccc
      781 gatcagggct tgaagaagaa ccctatgcaa aatcaggagg gagctgagtg catgcttttc
      841 atggacggtc tctacgccag catgggcttt tctccattca agggtgggcg cgccgaccca
      901 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
      961 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1021 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1081 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1141 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1201 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1261 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1321 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1381 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1441 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     1501 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1561 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     1621 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     1681 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     1741 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     1801 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     1861 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     1921 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     1981 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     2041 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     2101 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2161 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2221 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2281 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2341 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2401 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2461 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     2521 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2581 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2641 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2701 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2761 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2821 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2881 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2941 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3001 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     3061 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     3121 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     3181 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     3241 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     3301 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
