LOCUS       sequence-13085-e        3816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1399-HQ858731
FEATURES             Location/Qualifiers
     source          1..3816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      540..549
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1373..1472)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1489..1508)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1490..1508)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1513..1529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1513..1529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1631..1650)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1642..2451
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1709..1728)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2598..3186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3087..3106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3340..3357
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3516..3543)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3635..3721)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3770..3792
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3784..3801
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3785..3801
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggtggcc gaggcggaag tcatgcatca gtcccccgtt
      181 ccggtgctgg aggtgcagta ccaccggcgc gcgacgacca agggggttga cgacgtcgtc
      241 gggatgtccg cgcccgcccc cgcggaggtt ggggttgggg ttggggttga ggtcgaggtc
      301 gaggtcgagg tcgccgtgga ggtgcctcgc atggggttgg agcagcctga tgtcgcacca
      361 agtttatcca cggacatgat acagcagttt gtgcccaaca tccgttctgg tagcttcgcc
      421 gacattgggc ctcggaggta tatggaggac gagcacatcc gaatagatga tctttccgcc
      481 catctcggct cgatgttggt ctgccctctg cctagcgcct tctacggggt ctttgacggc
      541 catggcggcc cggacgccgc agcctacatg aagaggcatg cgatgaggtt cttgtttgag
      601 gaaagcgagt tcccacaggc gtcgcaggtg gatgaggtgt acctcgagtc cgtggagagc
      661 tgtgtccgca gagccttctt gcaggcggac cttgccctgg ctgatgatct ggacatcagc
      721 cggtcctctg gaaccacggc gctcactgca ttagtgtttg gaaggcaact ggtggtcgcg
      781 aacacggggg actgccgcgc ggtgttgtgc cggaaaggag tggcgatgga gatgtcccgc
      841 gaccaccgcg cgaaccacgt ggaggagcgc gagagggtgg ccgcgtcggg cgggtacatc
      901 gaggacgggt acctgaacgg ggtgctgtcg gtgacgcggg cgctgggcga ctgggacatg
      961 aaggcgcccg gcgcgtcggc gtcggcggcg ctgatcgcgg agcccgagtt ccggcgggcg
     1021 acgctgggtg aggacgacga gttcctcgtc atggggtgcg acgggatctg ggacgtgatg
     1081 acgagccagc acgcggtgag cctggtgcgg cgcggcctgc ggcagcacga cgaccccgcg
     1141 cgctgcgccc gggagctggt gatggaggcc aagcggctgg agacggccga caacctgacg
     1201 gtcgtcgtgg tgtgcttcgc gccggagatg gggtcgccgt ttacaccgca gcaacagcag
     1261 gaggaggagc tgcccgcgag gcccaggagc tgcaagaagg gcctgtcgac ggaggcgctg
     1321 tgcagcctga ggagctggct ggatacggac caccggaagg gtgggcgcgc cgacccagct
     1381 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     1441 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     1501 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1561 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1621 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1681 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1741 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1801 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1861 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1921 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     1981 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2041 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2101 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2161 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2221 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2281 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2341 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2401 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2461 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2521 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2581 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2641 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2701 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     2761 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2821 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2881 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2941 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     3001 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3061 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3121 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3181 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3241 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3301 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3361 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3421 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     3481 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3541 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3601 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3661 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     3721 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     3781 cgttgtaaaa cgacggccag tcttaagctc gggccc
//
