LOCUS       sequence-13086-e        3318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1400-HQ858732
FEATURES             Location/Qualifiers
     source          1..3318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(875..974)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(991..1010)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(992..1010)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1015..1031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1015..1031)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1133..1152)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1144..1953
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1211..1230)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2100..2688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2589..2608
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2842..2859
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3018..3045)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3137..3223)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3272..3294
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3286..3303
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3287..3303
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggaggcg gcagtcgcgg agacgcaggg gatgtcgccg
      181 tacgaggcgg cgcgggagcg gaacctgcag gagaacaagc gaaagatgga agccctcaac
      241 ctgcgccacc tctccgccgt catcaaggag gcccccaaga ccccttcccc catgaaaccg
      301 aagaggcgca ggatcatcga ggacgcggtc gtggttccct cgcctcccag gaggtcccgc
      361 cggcttgcca atgtccctga ggtcaagtac gcagagatag caccacaccg tgcagataga
      421 atgacaaggc ctcctagaag accagctgac ctcatctact tggcaagacg cggatcaatt
      481 tccatgaaag ctaggctgga ggcaacaagg aaggctgagg agctagaatc acaactcgat
      541 cctgaaatcc catccttcgt gaaggcgatg ttgcattcgc atgtggttcg aggcttttgg
      601 ctgggtctcc cgagccattt ctgtgacact tacatgccga agcaagacgc cactatcacc
      661 ttggtggatg agaaggatga agaattcgac accaactacc ttgcctacaa gaaggggcta
      721 agtggcgggt gggctggttt tgcgctatac catgcgattc aggatggaga ttcaactgtt
      781 tttcagttga ttaagcccac gaccttcaag gtgcatatcg tccgagcagc tactgatgat
      841 agcagtgaag tagatgagaa gggtgggcgc gccgacccag ctttcttgta caaagttggc
      901 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      961 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     1021 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     1081 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     1141 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     1201 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     1261 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     1321 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     1381 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     1441 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     1501 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     1561 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     1621 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     1681 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     1741 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     1801 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     1861 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     1921 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     1981 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     2041 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2101 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2161 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2221 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2281 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2341 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2401 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2461 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2521 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2581 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2641 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2701 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2761 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2821 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     2881 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     2941 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     3001 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     3061 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     3121 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     3181 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     3241 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     3301 agtcttaagc tcgggccc
//
