LOCUS       sequence-130861-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_COF1D02
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(546..645)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(662..681)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(663..681)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(686..702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(686..702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(804..823)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 tgattttttt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg cttcatggac aagaaatcct cgccggtgcg
      121 acgacaaaag cggcaggcga aggtgatcga ggagaacttc tgggactgca gcgtctgcac
      181 atatagaaac tcggcggagg ccttcaagtg tcgcatgtgc gacgtgcgga aaggaacctc
      241 cacgcgaaag ccacgcctga actcagcgct agtcgcccag caggccgcca ctttgccagg
      301 agccagtgtc aacatgccca acgggaagtc cgcctccgga tcacggcatg gcagtgggca
      361 cgaccggcag aggcacaagc ggtatccggc acggttgaaa aacgtggacc gatcgacggc
      421 acagacgcga gaggtgacgg tcaactcggt gaccgtgttc atcacagagt acaaggccaa
      481 gccggtgagc agccgacgcg aatccagtga gcagagcttc agcgagagca acgacagccg
      541 gagtgaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
      601 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
      661 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
      721 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
      781 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
      841 gaggccgcga ttaaattcca acatggatgc tga
//
