LOCUS       sequence-13088-e        2139 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Koushik SV, Vogel SS
  TITLE     Energy migration alters the fluorescence lifetime of Cerulean: 
            implications for fluorescence lifetime imaging Forster resonance 
            energy transfer measurements.
  JOURNAL   J Biomed Opt. 2008 May-Jun;13(3):031204. doi: 10.1117/1.2940367.
  PUBMED    18601528
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biomed 
            Opt. 2008 May-Jun;13(3):031204. doi: 10.1117/1.2940367."
COMMENT     Sequence Label: CTV
FEATURES             Location/Qualifiers
     source          1..2139
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1426..1428,1429..2139)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaacgccat cagcgacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc
      721 ggagagagcc tggagaagaa gacggccact tttgagaaca ttgtctgcgt cctgaacagg
      781 gaggtggaga gggtggccat gactgccgag gcctgcagcc ggcagcaccg gctggaccaa
      841 gacaagattg aagccctgag tagcaaggtg cagcagctgg agaggagcat tggcctcaag
      901 gacctggcga tggctgactt ggagcagaag gtcttggaga tggaggcatc cacctacgat
      961 ggggtcttca tctggaagat ctcagacttc gccaggaagc gccaggaagc tgtggctggc
     1021 cgcatacccg ccatcttctc cccagccttc tacaccagca ggtacggcta caagatgtgt
     1081 ctgcgtatct acctgaacgg cgacggcacc gggcgaggaa cacacctgtc cctcttcttt
     1141 gtggtgatga agggcccgaa tgacgccctg ctgcggtggc ccttcaacca gaaggtgacc
     1201 ttaatgctgc tcgaccagaa taaccgggag cacgtgattg acgccttcag gcccgacgtg
     1261 acttcatcct cttttcagag gccagtcaac gacatgaaca tcgcaagcgg ctgccccctc
     1321 ttctgccccg tctccaagat ggaggcaaag aattcctacg tgcgggacga tgccatcttc
     1381 atcaaggcca ttgtggacct gacagggctc tccggactca gatctgtgag caagggcgag
     1441 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1501 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1561 ctcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctcggc
     1621 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     1681 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1741 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1801 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1861 aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     1921 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     1981 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     2041 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2101 gccgccggga tcactctcgg catggacgag ctgtacaag
//
