LOCUS       sequence-13091-e        3912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1432-HQ858734
FEATURES             Location/Qualifiers
     source          1..3912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(1469..1568)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1585..1604)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1586..1604)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1609..1625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1609..1625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1727..1746)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1738..2547
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1805..1824)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2694..3282
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3183..3202
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3436..3453
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3612..3639)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3731..3817)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3866..3888
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3880..3897
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3881..3897
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggaatgc gtggagctgc tggattctcc cgatctcttc
      181 ttcctggacg gcggcgatgc gcatgcgacc gacttctcac tggagaacca tctcgagtca
      241 agtagctcgg caggtgccaa aaacaaagct gcgccgggtc atagtcatat tcatagtcat
      301 agtcatagcc ggcgtgctca gcttcatcgg agtaacgcgc ctgttccacc gccgctgcct
      361 ggaacctctt attatggcgc gcggaggaag gcgaaggccg cctgtcgccg tgtccccgaa
      421 ggcgtcctgg attcctggga caggttgttc ttggagggat accagtctga cctccgtgtc
      481 tccacagacg gcggcgctga gatttcgtcg cattcctgcg ttctcggcgt caaatcccct
      541 gtcctgagag ctatgctgga ggaagctaga ctgagagatg ggtttcggtg tatccgtgtc
      601 tctggcgcgc cttcggaagc agtccgtgtc ttcatcagat ttctctactc ttcacggttt
      661 gagcaggaac agatgaagga gcacgctctg catttgcttg tgctctccca cgtcttctcg
      721 gtgccgtctc tgaagacggt ctgcgtcgac caactcgaga ggggcttcct cgctactgac
      781 aacgtggtag acatgctgca gcttgccggg ctgtgtgacg cgccccgcct cgcgctcgcc
      841 tgtgcccgga tggtcatcgg ggatttcaag accatctctc tgacggatgg gtggaaagtg
      901 atgagacgga ccaatccaag tatggaacag gagctgctcg agtctcttgt cgaagctgat
      961 acgagaaggc aggaaagagc taaaaggatg gaagaaaaga aagtgtatct gcaactgtac
     1021 gaagcaatgg aggcgctggt ccacatctgc cgggacggat gcaggacgat cgggcctcga
     1081 gatcaagcgc tcaagggcag cctagcagcc gcctgcaagt ttcgcgcctg caagggcatt
     1141 gagctgctcg tgcgccactt ctcggcctgc ggagtccggg tgcccggcgg ctgtgccaac
     1201 tgcaggcgga tgtggcagct cctcgagctg cattcccgca tgtgcttcct cccggacacc
     1261 tgcaaggttc ctctctgcag gcattttaag gagaagatgc ggaatctggg gaggaaggaa
     1321 gagaccaaat ggaacctttt ggtgtgcaag gtgctggagc gcggaggaac catgggtttt
     1381 atgcccggaa ggagaaaaaa tttcatcaac cctaaggcca gctgggtgtt gccacgtggc
     1441 tcctcttgta ttaagggtgg gcgcgccgac ccagctttct tgtacaaagt tggcattata
     1501 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     1561 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     1621 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1681 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     1741 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     1801 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     1861 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1921 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     1981 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     2041 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     2101 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     2161 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     2221 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     2281 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     2341 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     2401 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     2461 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     2521 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     2581 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     2641 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2701 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2761 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2821 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2881 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2941 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3001 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3061 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     3121 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3181 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3241 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3301 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3361 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3421 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3541 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     3601 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     3661 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     3721 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3781 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     3841 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     3901 aagctcgggc cc
//
